Optical Filters
CoolLED recommends that excitation filters are used with its LED excitation products. Although LEDs produce a narrowband of excitation, there can be a small "tail" of excitation to shorter and longer wavelengths which may be undesirable for some applications.
Typically, if you have existing filters, these can be used with your CoolLED system. We are happy to discuss this with you and provide our recommendations to assist you to achieve the best LED wavelength - filter combination.
The tables below provide information on commercially available filters - and filter sets - matched to CoolLED's range of LED excitation wavelengths. These are regularly updated as new LED wavelengths become available. By clicking on a selected filter, the link will take you through to the suppliers website where you can view spectral details of the filter.
Multiband Filter sets
- Full multiband sets. Three piece set consisting of dual band excitation, emission and dichroic mirror.
- Pinkel sets. Four piece set consisting of 2 separate single band excitation filters and single dual band emission filter and dichroic mirror.
- Sedat sets. Five piece sets consisting of 2 separate single bandpass excitation filters, 2 separate single bandpass emission filters and a single dual band dichroic mirror.
| Multibandpass sets | ||||
| LED peak λ |
Common Fluorophore |
Chroma | Omega | Semrock |
| DAPI/FITC or DAPI/GFP |
DA/FI-A 380 & 470nm or 380 & 490nm |
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| DAPI/TRITC | ||||
| CFP/YFP | 59017 425nm or 440nm & 490nm or 500nm |
CFP/YFP-A 425 & 500nm |
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| GFP & DsRed or TRITC |
GFP/DsRed-A 470nm & 550nm |
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| FITC/Texas Red or GFP/mCherry |
59022 470nm or 490nm & 565nm or 585nm |
FITC/TxRed-A 470nm & 585nm or 490nm & 585nm |
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| Cy3/Cy5 | Cy3/Cy5-A 535nm & 635nm |
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| DAPI FITC TRITC |
69000 400nm & 470nm or 490nm & 550nm |
DA/FI/TR-A 380nm & 470nm & 550nm |
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| DAPI FITC Texas Red |
69002 400nm &490nm & 565nm or 585nm |
DA/FI/TX-B 400nm & 490nm & 565nm or 585nm |
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| Pinkel sets | ||||
| LED peak λ |
Common Fluorophore |
Chroma | Omega | Semrock |
| DAPI/FITC or DAPI/GFP |
DA/FI-2X-A 380nm & 490nm |
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| DAPI/TRITC | ||||
| CFP/YFP | 59217 425nm or 440nm & 500nm |
CFP/YFP-2X-A 425nm & 500nm |
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| GFP & DsRed or TRITC |
GFP/DsRed-SX-A 470nm & 550nm |
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| FITC/Texas Red or GFP/mCherry |
59222 470nm & 565nm or 585nm |
FITC/TXRED-2X-A 470nm & 585nm GFP/HcRed-2X-A 470nm & 585nm |
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| Cy3/Cy5 | CY3/CY5-2X-B 535nm or 550nm & 635nm |
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| DAPI FITC or GFP TRITC or Cy3 |
62000v3 365nm & 470nm or 490nm & 550nm |
DA/FI/TR-3X-A 380nm & 470nm & 550nm |
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| DAPI FITC or GFP Texas Red |
69302 400nm & 490nm & 565nm or 585nm |
BFP/GFP/HcRed-3X-A 365nm or 380nm & 470nm & 585nm DA/FI/TX-3X-A 380nm & 490nm & 565nm or 585nm |
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| CFP YFP TxRed or mCherry |
69308 425nm or 440nm & 500nm & 565nm or 585nm |
CFP/YFP/HcRed-3X-A 425nm & 500nm & 585nm |
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| Sedat sets | ||||
| LED peak λ |
Common Fluorophore |
Chroma | Omega | Semrock |
| DAPI/FITC or DAPI/GFP |
DA/FI-2X2M-A 380nm & 490nm |
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| DAPI/TRITC | ||||
| CFP/YFP | 89002 425nm or 440nm & 490nm or 500nm |
CFP/YFP-2X2M-A 425nm & 500nm |
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| GFP & DsRed or TRITC |
89019 470nm or 490nm & 550nmm |
GFP/DsRed-2X2M-B 470nm & 550nm |
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| FITC/Texas Red & Red or mCherry |
89017 490nm & 565nm or 585nm 89021 470nm & 565nm or 585nm |
FITC/TXRED-2X2M-A 490nm & 585nm or 595nm |
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| Cy3/Cy5 | CY3/CY5-2X2M-B 525nm or 535nm or 550nm & 635nm |
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| DAPI FITC TRITC |
DA/FI/TR-3X3M-A 380nm & 470nm or 490nm & 550nm |
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| DAPI FITC Texas Red |
89014 400nm & 490nm & 565nm or 585nm |
DA/FI/TX-3X3M-A 380nm & 490nm & 565nm or 585nm |
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| CFP YFP mCherry |
89006 440nm & 490nm or 500nm & 565nm or 585nm |
CFP/YFP/HcRed-3X3M-A 425nm & 500nm & 585nm |
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| FRET sets | ||||
| LED peak λ |
Common Fluorophore |
Chroma | Omega | Semrock |
| BFP/GFP | FRET-BFP/GFP-A 380nm |
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| CFP/YFP | 49052 440nm |
FRET-CFP/YFP-A 440nm |
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| GFP/RFP | XF151-2 470nm |
FRET-GFP/RFP-A 470nm |
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| FISH sets | ||||
| LED peak λ |
Common Fluorophore |
Chroma | Omega | Semrock |
| DAPI | DAPI-1160A 380nm |
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| SpectrumAqua | SpAqua-A 425nm or 440nm |
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| SpecrtumGreen | SpGr-A 490nm |
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| SpectrumGold | SpGold-A 525nm or 535nm |
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| SpectrumOrange | SpOr-A 535nm or 550nm |
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| SpectrumRed | SpRed-A 585nm |
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| Cy5 SpectrumFRed |
CY5-4040A 635nm |
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| Cy5.5 | CY5.5-A 635nm |
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| Cy7 | CY7-A 700nm |
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